Heparinase I – Research Grade PN 50-010 and 50-010-001
| Heparinase I - Diagnostic Grade Datasheet | Heparinase I - Research Grade Datasheet |
| Synonyms | Heparinase; heparin lyase; heparin eliminase |
| Source | Flavobacterium heparinum (recombinant) |
| EC Number | 4.2.2.7 |
| CAS_Number | 9025-39-2 |
| Catalyzed Reaction | The enzyme cleaves selectively, via an elimination mechanism, highly sulfated polysaccharide chains containing 1-4 linkages between hexosamines and O-sulfated iduronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III binding pentasaccharide domain in the heparin molecule. |
| Substrate Specificity | Heparin; heparan sulfate (specific activity with heparin is approx. 3 times higher than with heparan sulfate). |
| Properties | Molecular weight: 42,508 Da |
| Isoelectric point: 9.3 – 9.5 | |
| pH optimum for activity: 6.5 – 7.5 | |
| pH range for activity: 4 – 9 | |
| Optimal testing temperature range: 20°C – 37°C | |
| Optimal storage temperature: – 70°C | |
| Purity |  |
| Specific Activity | 90-110 IU/mg using the following Unit definition: One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 µmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30° C and pH 7.0. |
| Some other companies define one Unit (U) as " 1U forms 0.1 µmole of unsaturated uronic acid per hour at 25°C and pH 7.5; 1 IU is equivalent to 600 U. | |
| Stability | Expiration is primarily established at 3 years after manufacturing. The Heparinase I is kept frozen at -70 ° C in aqueous buffer containing Sodium Phosphate or Sucrose 5%. |
| Applications | For the neutralization of heparin in blood and plasma samples before analysis. |
| For similar in vitro neutralization of low molecular weight heparins. | |
| As integral part of in vitro diagnostic tests for the neutralization of heparin (blood clotting tests, platelet tests). | |
| In blood collection tubes for the neutralization of heparin. | |
| For the preparation of low molecular weight heparins from unfractionated heparin. | |
| As a research reagent (glycosaminoglycan degradation) | |
| For the preparation of disaccharides of heparin and the preparation of oligosaccharide libraries. |
