Heparinase High Purity Enzymes for Glycobiology Research

IBEX has developed a proprietary expression system for the production of our enzymes in Flavobacterium heparinum. This system allows for the production of each glycosaminoglycan enzyme in a way that minimizes the cross contamination of other GAG enzymes which can occur when enzymes are produced from the native strain of Flavobacterium heparinum (Pedobacter heparinus). When combined with our well-defined proprietary purification process our enzymes are of the highest purity.

Due to its high purity and reaction time IBEX Heparinase I is the only heparinase used in FDA and EU approved hemostasis-measuring Point Of Care devices.

Heparinase I

Synonyms: Heparinase; heparin lyase; heparin eliminase
Source: Flavobacterium heparinum (recombinant)

Catalyzed Reaction: The enzyme cleaves selectively, via an elimination mechanism, highly sulfated
polysaccharide chains containing 1-4 linkages between hexosamines and Osulfated iduronic acid residues. The reaction yields oligosaccharide products
(mainly disaccharides) containing unsaturated uronic acids which can be
detected by UV spectroscopy at 232 nm. The enzyme also cleaves the
antithrombin III binding pentasaccharide domain in the heparin molecule.

Substrate Specificity: Heparin; heparan sulfate (specific activity with heparin is approx. 3 times higher
than with heparan sulfate).

Heparinase

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    Heparinase II

    Synonyms: Heparitinase
    Source: Flavobacterium heparinum (recombinant)

    Catalyzed Reaction: The enzyme cleaves, via an elimination mechanism, sulfated polysaccharide
    chains containing 1-4 linkages between hexosamines and uronic acid residues
    (both iduronic and glucuronic acid residues). The reaction yields oligosaccharide
    products (mainly disaccharides) containing unsaturated uronic acids which can
    be detected by UV spectroscopy at 232 nm. The enzyme cleaves both heparin
    and heparan sulfate, with the heparan sulfate activity being about twice as high
    as the heparin activity.

    Substrate Specificity: Heparin, heparan sulfate.

    Heparinase III

    Synonyms: Heparitinase

    Source: Flavobacterium heparinum (recombinant)

    Catalyzed Reaction: The enzyme cleaves, via an elimination mechanism, sulfated polysaccharide chains containing 1-4 linkages between hexosamines and uronic acid residues (both iduronic and glucuronic acid residues). The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme cleaves both heparin and heparan sulfate, with the heparan sulfate activity being about twice as high as the heparin activity.

    Substrate Specificity: Heparin, heparan sulfate.

    Click the .pdf icons below to view the complete product Data Sheet

    Heparinase
    Heparinase I
    Heparinase
    Heparinase II
    Heparinase
    Heparinase III

    IBEX produces high purity enzyme products that do not contain preservative agents or proteinaceous stabilizers such as albumins.

    Our enzymes are sold in either lyophilized  or frozen liquid form. Enzymes formulated as  liquids are shipped on dry ice. Each shipment is accompanied by a certificate of analysis.

    IBEX sells its enzymes in International Units (IU).  One international unit is defined as “the amount of enzyme that will liberate 1.0 µmole per minute of unsaturated oligosaccharides from substrates at 30°C and pH 7.0 (Heparinase I), 7.5 (Heparinase II and III)”.

    Please note that IBEX uses International Units (IU) as a definition of units while some suppliers have a definition of a “Unit” which reports the digestion capacity in hours which translates  to being several hundred times less than an International Unit.  This is important when comparing prices and specific activity.  IBEX has standardized it’s enzymatic activity measurement over two minutes and reports the result in activity per mL obtained in one minute.

    IBEX heparinases are now available in Freeze-Dried Powder form!